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12,13 If analysis of the pellet indicates the presence of a substantial amount of the desired protein, the fusion protein may often be recovered by resuspending the pellet in a solution comprising 1.5% N-lauroylsarcosine, 25 mM triethanolamine and 1 mM EDTA, pH 8.0, mixing for 10 minutes and then recentrifuging at 10,000 g for 10 minutes at 4C

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Nature 510 , 370375 (2014)